Villanelle (played by Jodie Comer) is one of the main characters in the BBC America spy comedy-thriller series, Killing Eve.

Villanelle is a brilliant and darkly funny but manipulative assassin who uses her charms and talent for accents to deceive her victims with stylish eccentricity. Her bisexuality is made clear from the very beginning when she is briefly shown in the middle of a threesome with a man and a woman. 

She is repeatedly shown having and enjoying sex with men and women. Her main fascination throughout the series is, of course, Eve Polastri (Sandra Oh), an MI5 analyst turned agent. She spends the series playing a complex game of cat and mouse with Eve who is also hunting her down.

Villanelle displays many of the most negative stereotypes about bisexuality. She uses her sexuality as a weapon, is untrustworthy, and mentally unstable. However, as the seasons advance, she is undeniably charming and her relationship with Eve is surprisingly complex. She also lives in a universe full of well-rounded queer characters, many of whom are not villains.